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smart race cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa smart race cdna synthesis kit
    Smart Race Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6075 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart+race+cdna+synthesis+kit/SMARTer+PCR+cDNA+Synthesis+Kit/pm36495771-103-6-11
    Average 96 stars, based on 6075 article reviews
    smart race cdna synthesis kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Nile tilapia DNA sensor STING is involved in the IFN-β and AP-1 signaling pathways in the immune response dependent on DDX41.
    Article Snippet: Stimulator of interferon genes (STING) plays important roles in innate immunology.. In this study, we isolated the STING gene in Nile tilapia, termed OnSTING.. Using quantitative RT–PCR, we explored the expression patterns of the OnSTING gene.

    Article Title: Fig fruit ripening is regulated by the interaction between ethylene and abscisic acid.
    Article Snippet: Fleshy fruit ripening is typically regulated by ethylene in climacteric fruits and abscisic acid (ABA) in non‐climacteric fruits.. Common fig (Ficus carica) shows a dual‐ripening mechanism, which is not fully understood.. Here, we detected separate peaks of ethylene and ABA in fig fruits at the onset‐ and on‐ ripening stages, in conjunction with a sharp rise in glucose and fructose contents.

    Amplification:

    Article Title: Prominins control ciliary length throughout the animal kingdom: New lessons from human prominin-1 and zebrafish prominin-3
    Article Snippet: As a result, a 1.2-kb cDNA fragment was obtained, subcloned into pCRII-TOPO TA vector (Invitrogen), and sequenced. .. The missing extremities were amplified by 5′- and 3′-RACEs using a SMART RACE cDNA synthesis kit (catalog no. 634914, Clontech) according to the manufacturer's instructions. .. The gene-specific oligonucleotides used for the first and second round of amplification of 5′-RACE were 5′-GATGAAGTGTAGTCCACAAGGGTGCGTTCTG-3′ (jj-593) and 5′-ACTAACATGAAGATCCAGGAGATCAG-3′ (jj-594).

    cDNA Synthesis:

    Article Title: Prominins control ciliary length throughout the animal kingdom: New lessons from human prominin-1 and zebrafish prominin-3
    Article Snippet: As a result, a 1.2-kb cDNA fragment was obtained, subcloned into pCRII-TOPO TA vector (Invitrogen), and sequenced. .. The missing extremities were amplified by 5′- and 3′-RACEs using a SMART RACE cDNA synthesis kit (catalog no. 634914, Clontech) according to the manufacturer's instructions. .. The gene-specific oligonucleotides used for the first and second round of amplification of 5′-RACE were 5′-GATGAAGTGTAGTCCACAAGGGTGCGTTCTG-3′ (jj-593) and 5′-ACTAACATGAAGATCCAGGAGATCAG-3′ (jj-594).

    Article Title: The calcium channel β2 (CACNB2) subunit repertoire in teleosts
    Article Snippet: .. RACE Ready cDNA was created using the SMART RACE cDNA synthesis kit (Clontech, Palo Alto, CA). ..

    Article Title: Establishment and application of multiple immunoassays for environmental estrogens based on recombinant Japanese flounder (Paralichthys olivaceus) choriogenin protein.
    Article Snippet: Environmental estrogens have generated great concern because of their potential threat to aquatic organisms; however, the commonly used vitellogenin (Vtg) biomarker detection methods are not capable of detecting estrogenic activity below 10 ng/L 17β-estradiol.. In this study, we developed multiple immunoassays based on Japanese flounder (Paralichthys olivaceus) choriogenin (Chg), a highly sensitive biomarker of environmental estrogens.. Chg genes (ChgL and ChgH) of Japanese flounder were cloned for the first time, and a recombinant ChgL protein with a molecular weight of approximately 52 kDa was prepared using a prokaryotic expression system and purified using Ni-affinity column chromatography.

    Article Title: Susceptibility towards Enterotoxigenic Escherichia coli F4ac Diarrhea Is Governed by the MUC13 Gene in Pigs
    Article Snippet: Total RNA was extracted from the jejunum of both adhesive and non-adhesive F 2 animals using the Rneasy Fibrous Tissue Mini Kit (Qiagen). .. The first strand-complementary DNA was synthesized with the SMART RACE cDNA synthesis Kit (Clontech) and the 5′-Full RACE Kit (TaKaRa). .. To obtain the extended 5′-end of MUC13 cDNA, the first strand cDNA (Clontech) was first amplified with MUC13 nested primers F3/NF3 ( ) and universal primers UPM/NUP (Clontech, ).

    Article Title: A Novel Splicing Variant of Peroxisome Proliferator-Activated Receptor-γ ( Pparγ1sv ) Cooperatively Regulates Adipocyte Differentiation with Pparγ2
    Article Snippet: .. 5′- and 3′-Ready SMART cDNA was synthesized from 1 μg of total RNA using the SMART RACE cDNA synthesis kit according to the manufacturer’s instructions (Takara Bio). .. The 5′-end of mouse Pparγ cDNA was amplified from 5′-Ready SMART cDNA using mPPARg_5RACE_LP2, 5′-TTGGGTCAGCTCTTGTGAATGGAATG-3′ and Universal Primer Mix (UPM) (Takara Bio).

    Reverse Transcription:

    Article Title: Establishment and application of multiple immunoassays for environmental estrogens based on recombinant Japanese flounder (Paralichthys olivaceus) choriogenin protein.
    Article Snippet: Environmental estrogens have generated great concern because of their potential threat to aquatic organisms; however, the commonly used vitellogenin (Vtg) biomarker detection methods are not capable of detecting estrogenic activity below 10 ng/L 17β-estradiol.. In this study, we developed multiple immunoassays based on Japanese flounder (Paralichthys olivaceus) choriogenin (Chg), a highly sensitive biomarker of environmental estrogens.. Chg genes (ChgL and ChgH) of Japanese flounder were cloned for the first time, and a recombinant ChgL protein with a molecular weight of approximately 52 kDa was prepared using a prokaryotic expression system and purified using Ni-affinity column chromatography.

    Rapid Amplification of cDNA Ends:

    Article Title: Establishment and application of multiple immunoassays for environmental estrogens based on recombinant Japanese flounder (Paralichthys olivaceus) choriogenin protein.
    Article Snippet: Environmental estrogens have generated great concern because of their potential threat to aquatic organisms; however, the commonly used vitellogenin (Vtg) biomarker detection methods are not capable of detecting estrogenic activity below 10 ng/L 17β-estradiol.. In this study, we developed multiple immunoassays based on Japanese flounder (Paralichthys olivaceus) choriogenin (Chg), a highly sensitive biomarker of environmental estrogens.. Chg genes (ChgL and ChgH) of Japanese flounder were cloned for the first time, and a recombinant ChgL protein with a molecular weight of approximately 52 kDa was prepared using a prokaryotic expression system and purified using Ni-affinity column chromatography.

    Synthesized:

    Article Title: Susceptibility towards Enterotoxigenic Escherichia coli F4ac Diarrhea Is Governed by the MUC13 Gene in Pigs
    Article Snippet: Total RNA was extracted from the jejunum of both adhesive and non-adhesive F 2 animals using the Rneasy Fibrous Tissue Mini Kit (Qiagen). .. The first strand-complementary DNA was synthesized with the SMART RACE cDNA synthesis Kit (Clontech) and the 5′-Full RACE Kit (TaKaRa). .. To obtain the extended 5′-end of MUC13 cDNA, the first strand cDNA (Clontech) was first amplified with MUC13 nested primers F3/NF3 ( ) and universal primers UPM/NUP (Clontech, ).

    Article Title: A Novel Splicing Variant of Peroxisome Proliferator-Activated Receptor-γ ( Pparγ1sv ) Cooperatively Regulates Adipocyte Differentiation with Pparγ2
    Article Snippet: .. 5′- and 3′-Ready SMART cDNA was synthesized from 1 μg of total RNA using the SMART RACE cDNA synthesis kit according to the manufacturer’s instructions (Takara Bio). .. The 5′-end of mouse Pparγ cDNA was amplified from 5′-Ready SMART cDNA using mPPARg_5RACE_LP2, 5′-TTGGGTCAGCTCTTGTGAATGGAATG-3′ and Universal Primer Mix (UPM) (Takara Bio).



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